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Chapter 2Theme 1 · Cells and the Chemistry of LifeG2 / G3

Sec 3 Combined Science (Biology) Movement of Substances notes

Cells never sit still — the particles dissolved in and around them are constantly jostling, and that random jostling moves substances without any cell spending effort. Diffusion carries oxygen into your blood, carbon dioxide out of a leaf, and digested food into your gut lining, each particle drifting from where it is common to where it is rare. Osmosis tells the same story for water molecules only: water crosses a cell's partially permeable membrane towards the more concentrated solution, firming up plant tissue, swelling animal cells and — when the water goes the other way — leaving lettuce limp and cheek cells shrivelled.

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Overview

What you’ll learn

  • Define diffusion and describe its roles in nutrient uptake and gaseous exchange in plants and humans.
  • Define osmosis and describe the effects of osmosis on plant and animal tissues.

Key points

  1. 1Diffusion is the net movement of particles from a region where they are more concentrated to where they are less concentrated (down a concentration gradient).
  2. 2Diffusion happens because particles move randomly all the time; it needs no energy from the cell and works fastest when the gradient is steep, the distance short and the temperature higher.
  3. 3Diffusion roles: oxygen into the blood and carbon dioxide out in the lungs; gases in and out of leaves through the stomata; digested food products absorbed at the small-intestine lining.
  4. 4Osmosis is the diffusion of water from a dilute solution to a more concentrated solution across a partially permeable membrane.
  5. 5Plant tissue in water: cells gain water and become firm (the wall stops them bursting). Plant tissue in a concentrated solution: cells lose water and the tissue goes limp.
  6. 6Animal cells have no wall: in dilute solutions they swell and may burst; in concentrated solutions they shrink.

Notes · 1 of 5

Why substances move by themselves

Random particle motion produces one-way net movement.

Dissolved particles and gas particles never stop moving — they jiggle randomly, colliding and ricocheting billions of times a second. Watch any single particle and its path is a random walk with no destination. But watch the crowd: where a substance is crowded (high concentration) more particles happen to leave than arrive, and where it is sparse (low concentration) more arrive than leave. The result is a net movement from high to low concentration — no cell needs to push anything.

Three conditions make this net movement faster: a steeper concentration difference (bigger crowd gap), a shorter distance to travel, and a higher temperature (faster jiggling). Three everyday proofs: a tea bag colours hot water faster than cold; a smell crosses a room in seconds through air but takes far longer through a closed door; and a sugar cube dissolves and spreads quickest in stirred hot tea.

Notes · 2 of 5

Diffusion defined

The definition, and the everyday roles that keep organisms alive.

Diffusion is the net movement of particles from a region of higher concentration to a region of lower concentration, down a concentration gradient. Three words carry the marks: net (overall drift, not every single particle), higher to lower (the direction), and particles (any dissolved substance or gas — not just water).

Life runs on diffusion. In human nutrition, the end-products of digestion — glucose and amino acids — are in high concentration inside the small intestine after a meal and lower concentration in the blood, so they diffuse across the intestinal lining into the bloodstream. In gaseous exchange in humans, oxygen is crowded in the lungs' air sacs and sparse in the blood, so it diffuses in; carbon dioxide runs the opposite way, out. In plants, gases move in and out of leaves through tiny pores called stomata: carbon dioxide diffuses in for photosynthesis by day, and oxygen diffuses out as a product.

Diffusion at work
WhereSubstanceDirection of net movement
lung air sac → bloodoxygeninto the blood (high in air sac, low in blood)
blood → lung air saccarbon dioxideout of the blood
small intestine → bloodglucose, amino acidsinto the blood after a meal
leaf air space → airoxygen, water vapourout through open stomata
air → leafcarbon dioxidein through open stomata (day)

Notes · 3 of 5

Osmosis defined

Water's special journey across a partially permeable membrane.

Osmosis is the diffusion of water molecules from a dilute solution to a more concentrated solution through a partially permeable membrane. Two upgrades turn diffusion into osmosis: only water moves, and it must cross a partially permeable membrane — a barrier with tiny holes that let small water molecules through but hold back larger dissolved particles. The membrane is what makes osmosis matter to cells, because every cell is wrapped in exactly such a membrane.

Direction matters and is the most-tested idea in the topic: water moves from the dilute (more watery) side to the concentrated (less watery) side. Say it as "water goes where the dissolved substances are more crowded" and the direction can never be reversed by a trick question. A simple model: a tube of 2% sugar solution and a tube of 8% sugar solution joined by a partially permeable membrane — water crosses from the 2% side into the 8% side, so the 8% level rises and dilutes.

Notes · 4 of 5

Osmosis in plant tissues

Why watering makes plants firm and drought makes them limp.

A plant cell's cell sap (the solution in its large vacuole) is usually more concentrated than the water around the roots or in a raindrop, so water tends to enter by osmosis. As the vacuole swells it pushes the cytoplasm against the rigid cellulose wall, and the cell becomes firm. That internal push, cell after cell, is what keeps stems upright and lettuce crisp. The wall is the plant's safety device: it is strong, so a firm plant cell does not burst even in pure water.

Reverse the surroundings and the plant loses. In a concentrated solution — salty or sugary water, or a plant left without water so its cell sap becomes relatively concentrated — water leaves the cells by osmosis. Vacuoles shrink, the cytoplasm pulls away from the wall, and the tissue goes limp: wilting leaves, drooping stems. The wall itself never disappears; only the water content changes.

Plant cells and their surroundings
Surrounding solutionWater movementVisible effect
dilute (e.g. pure water)into the cellcell becomes firm; tissue rigid
same concentration as cell sapno net movementcell stays as it is
concentrated (e.g. salt/sugar solution)out of the cellvacuole shrinks; tissue goes limp, plant wilts

Notes · 5 of 5

Osmosis in animal tissues

No wall, no protection: swelling, shrinking and the body's balancing act.

Animal cells have no cell wall, so osmosis has free rein over their size. Place a red blood cell or a cheek-lining cell in pure water: water floods in, the cell swells — and because nothing resists the inflow, it may burst. Place the same cell in a concentrated salt solution: water leaves, and the cell shrinks into a crinkled shape. Only in a solution matching the cell's own concentration — as body fluids normally do — does the cell keep its size.

This is why the fluids used in medicine and sport are matched to body concentration: drips and rehydration drinks are formulated so that cells neither swell nor shrink. It also explains kitchen observations — vegetables sprinkled with salt lose water and soften, because the salt solution outside is now more concentrated than the cell sap inside.

Revise

Key terms

The words examiners expect you to use precisely.

diffusion
The net movement of particles from a region of higher concentration to a region of lower concentration.Needs no energy from the cell.
concentration gradient
The difference in concentration between two regions; particles diffuse down it.Steeper gradient → faster diffusion.
net movement
The overall drift of a particle population, even though individual particles move randomly both ways.At equal concentrations net movement is zero.
osmosis
The diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane.A special case of diffusion involving water only.
partially permeable membrane
A barrier that lets water molecules pass but holds back larger dissolved particles.The cell membrane is one; it is what makes osmosis possible.
dilute solution
A solution with a low concentration of dissolved substances (much water).Water leaves a dilute solution during osmosis.
Show 4 more key terms
concentrated solution
A solution with a high concentration of dissolved substances (little water).Water enters a concentrated solution during osmosis.
cell sap
The solution of water and dissolved substances inside a plant cell's large vacuole.Usually more concentrated than the water outside the root.
stomata
Tiny pores, mostly on the underside of leaves, through which gases diffuse in and out.The route for gaseous exchange in plants.
gaseous exchange
The two-way diffusion of oxygen and carbon dioxide between an organism and its surroundings.Lungs in humans; stomata and leaf surfaces in plants.

Revise

Common mistakes

Where students most often lose marks in this chapter — and what to do instead.

  • Mistake: Defining diffusion as "the movement of water from dilute to concentrated".

    That definition is osmosis. Diffusion moves any particles, and its direction is high → low concentration.

    Instead: Diffusion: any particles, high → low concentration, no membrane required. Osmosis: water only, dilute → concentrated, through a partially permeable membrane.

  • Mistake: Writing the definition as "solute particles moving through the membrane".

    In osmosis the dissolved particles stay put; the water moves.

    Instead: Only water crosses the partially permeable membrane; the membrane holds back the dissolved substance.

Show 9 more mistakes
  • Mistake: Reversing the direction: "water moves from strong to weak solution".

    That is solute thinking applied to water.

    Instead: Water always moves dilute → concentrated, towards the more crowded dissolved particles.

  • Mistake: Leaving "partially permeable membrane" out of the osmosis definition.

    Without the membrane it is just diffusion — the membrane is what distinguishes osmosis.

    Instead: Full osmosis definition = water + dilute → concentrated + through a partially permeable membrane.

  • Mistake: Claiming particles stop moving once concentrations are equal.

    Particles keep moving in both directions forever; only the net movement becomes zero.

    Instead: Dynamic equilibrium: movement continues, equal both ways, no further change in concentration.

  • Mistake: Expecting a plant cell to burst in pure water.

    The rigid cellulose wall resists the swelling.

    Instead: Plant cells become firm in water but do not burst; animal cells (no wall) can burst.

  • Mistake: Saying animal cells stay firm when placed in water.

    Without a wall nothing stops the water entering.

    Instead: Animal cells swell in dilute solutions and may burst; they shrink in concentrated solutions.

  • Mistake: Forgetting temperature in "rate of diffusion" answers.

    Higher temperature speeds particle movement, so diffusion is faster.

    Instead: Three rate factors: steeper gradient, shorter distance, higher temperature.

  • Mistake: Writing that osmosis moves glucose or mineral salts.

    Only water molecules ever cross the partially permeable membrane during osmosis.

    Instead: Glucose and salts are the dissolved substances; they are what water moves towards.

  • Mistake: Explaining a wilted lettuce as "cells have lost their walls".

    Walls are rigid structures; they never dissolve or disappear.

    Instead: Wilting = vacuoles lose water by osmosis, so cells no longer press against their walls.

  • Mistake: Saying the net movement at equilibrium is "zero movement".

    Zero net movement is not zero movement.

    Instead: Particles move equally in both directions; concentrations stay constant.

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Define diffusion and describe its roles in nutrient uptake and gaseous exchange in plants and humans.

Diffusion is best defined as the net movement of particles

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5 multiple-choice questions and structured questions with answer lines. Chapter 2 (diffusion and its roles; osmosis and its effects on plant and animal tissues).

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Questions

Frequently asked questions

What is the difference between diffusion and osmosis?

Diffusion moves any particles from high to low concentration and needs no membrane. Osmosis moves water only, from a dilute to a more concentrated solution, and must cross a partially permeable membrane.

Why does water move towards the more concentrated solution?

Water particles drift down their own gradient — there is more water (relative to dissolved particles) on the dilute side, so water naturally shifts to the side where water is "rarer", which is the concentrated solution.

Why do plant cells not burst in pure water while animal cells can?

Plant cells have a rigid cellulose wall that resists the swelling, so they just become firm. Animal cells have no wall, so the extra water swells them until they may burst.

What speeds up diffusion?

Three things: a steeper concentration difference, a shorter distance to travel, and a higher temperature (faster-moving particles).

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