GCSE (9–1) · GCSE Biology (Foundation and Higher)
Chapter 3 Practice Paper
Enzymes and the digestive system
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Original practice material written for the MOE GCSE Biology syllabus. It is not an official MOE or school examination paper. Chapter 3: levels of organisation, enzyme action and denaturation, the digestive enzymes and their products, bile, the food-tests and amylase-pH required practicals, and rate calculations.
| For Examiner’s Use | |
|---|---|
| Section A | / 10 |
| Section B | / 20 |
| Total | / 30 |
Section A: Multiple-choice questions[10 marks]
Choose one answer for each question. Each question is worth 1 mark.
The active site of an enzyme is
Which sentence about denaturation is correct?
Bile is stored in the
Starch is digested into
A food sample turns Biuret reagent purple. The sample contains
Far from its optimum pH, an enzyme reaction slows to almost nothing because
A reaction finishes in 25 seconds. Its rate is
Which food test must be heated to show a positive result?
The products of fat digestion are absorbed mainly through
An organ system is
Section B: Structured questions[20 marks]
Answer all questions in the spaces provided.
An enzyme’s reaction rate was measured at four temperatures.
Rate data
| Temperature (°C) | Rate (arbitrary units) |
|---|---|
| 15 | 2 |
| 25 | 4 |
| 35 | 8 |
| 45 | 3 |
Identify the temperature that gave the highest rate in this experiment.
Explain why the rate rose between 15 °C and 35 °C.
Explain why the rate fell at 45 °C.
State why the true optimum may lie between 35 °C and 45 °C.
Solution X was tested with three food reagents: iodine stayed orange-brown, Benedict’s gave orange after heating, and Biuret stayed blue.
State which food X contains.
State what the iodine result shows.
State what the Biuret result shows.
Explain why the Benedict’s tube was heated.
This question is about bile and fat digestion.
Name the two organs bile passes through between being made and being released into the small intestine.
Describe what happens to fat droplets during emulsification and explain the effect on lipase.
State one further way bile helps digestion in the small intestine.
Two enzyme reactions were timed: reaction P finished in 100 s; reaction Q finished in 50 s.
Calculate the rate of each reaction, in per second.
State how many times faster reaction Q was.
State one change that would halve reaction P’s time.
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